Atcc hl-60

HL-60 cells (white arrow) and MDA-MB-231 cells (red arrow) passing through the magnetophoretic separation module under various focusing buffer flow rates and magnetic field strength conditions of (c) 3 mL/h and 0 mT, (d) 3 mL/h and 20 mT, and (e) 5 mL/h and 20 mT. (f – i) Enlarged images of cells during separation from Figure 2 e.

The doubling time is about 36–48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic … 1/1/2005 Organism Type: Human: This is a non-adherent human leukocyte cell line. ATCC # CCL-240: Tissues: Lymphocyte: Phenotype: Suspension: Primary: no: Application: Cell 7klvsurgxfwlvlqwhqghgiruoderudwru\uhvhdufksxusrvhvrqo\ ,wlvqrwlqwhqghgiruxvhlqkxpdqv :kloh$7&&xvhvuhdvrqdeohhiiruwvwrlqfoxghdffxudwhdqgxs wr Article Snippet: Utah), human promyelocytic leukemia HL-60 cells, drug-resistant HL-60/MX2 (from ATCC), human lung tumor drug-resistant NCI-H69/AR cells (from ATCC), human lung tumor A549 cells (from ATCC) and human epidermoid tumor KB cells (from ATCC) were maintained in RPMI-1640/PBS medium with 0.5M PBS, 2 mM L-glutamine, 5% penicillin–streptomycin, 10% … ATCC HL-60, Promyelocytic Leukemia, Frozen Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide … The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA). HL-60 cells were commonly cultured in RPMI 1640 medium supple-mented with 10% (v/v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2. Eos-HL-60 .

07.11.2020 Atcc hl-60

96100920 . Human promyelocytic leukaemic cells established from HL60 cells by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR . July 2018 There is no reported evidence by the originators of HL-60 (33), the American Type Culture Collection (ATCC) (Rock-ville, Md.), or the European Collection of Cell Cultures I got a HL-60 cells from ATCC and cultured them in IMEM high glucose+20% FBS ( non heat inactivated as recommend by atcc technical support)and cryopreserved the cells at a … DNA into HL-60 Human lymphoblasts (ATCC Cat. No. CCL-240) using Lipofectamine™ LTX Reagent (Cat. No. 15338-100). Important Guidelines for Transfection Follow these important guidelines when transfecting DNA into HL-60 cells using Lipofectamine™ LTX Reagent: Purpose. The screening procedure is called the NCI-60 Human Tumor Cell Lines Screen, and it is one of the Discovery & Development Services of NCI's Developmental Therapeutics Program (DTP).

7klvsurgxfwlvlqwhqghgiruoderudwru\uhvhdufksxusrvhvrqo\ ,wlvqrwlqwhqghgiruxvhlqkxpdqv :kloh$7&&xvhvuhdvrqdeohhiiruwvwrlqfoxghdffxudwhdqgxs wr

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals.

Aliases: HL-60. Disease ATCC Gender Female HL60 sequenced and profiled in following datasets. Loss-of-function screens Combined RNAi (Broad, Novartis, Marcotte)

I culture in 1640 RPMI The human acute promyelocytic leukemia HL-60 (ATCC® CCL-240™), Jurkat (human acute T lymphocytic leukemia, ATCC® TIB-152™) and K-562 (human chronic myeloge-nous leukemia, ATCC® CCL-243™) suspension cells were maintained in RPMI-1640, while HepG2 (human hepato-cellular carcinoma, ATCC® HB-8065™), MCF7 (human May 22, 2018 · The human promyelocytic leukemia cell line HL-60 (ATCC® CCL240™) was purchased from the American Type Culture Collection (ATCC, USA). HL-60 cells were commonly cultured in RPMI 1640 medium supplemented with 10% ( v /v) fetal bovine serum containing 100 U/ml penicillin and 100 U/ml streptomycin at 37 °C in a humidified atmosphere with 5% CO 2 .

Lung.

1. The day of transfection, count the cells to determine culture density. Plate 1 x 105 cells per well in 0.5 ml of HL-60 CELL LINE RC2DVQ0F6O Overview Names 3: Identifiers 2: Relationships 2: Characteristic Attributes 3: Notes 2: Audit Info References 3: Substance Class Jan 17, 1989 · Although intact ATCC HL-60 cells specifically and saturably take up 1,25-dihydroxy[3H]vitamin D-3, whole cell extracts have little or no specific binding of 1,25-dihydroxyvitamin D-3. The absence of specific binding can now be explained by the action of a serine proteinase in these cells. Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support. ATCC HL-60, Promyelocytic Leukemia, Frozen Manufacturer: ATCC CCL240 HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. Nov 29, 2014 · Strategies Cell culture and solutions HL 60 human myeloblastic leukemia cells derived through the original patient isolate, a generous gift of Dr. Robert Gallagher, were grown in RPMI 1640 supplemented with 5% fetal bovine serum and 1x antibiotic antimycotic in the 5% CO2 humidified environment at 37 C. The protocols I have read seem straightforward on differentiating HL-60 cells, however I am not having luck using 1.3% DMSO and/or 1uM ATRA (all trans retinoic acid).

If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. HL-60/MX2 is a mitoxantrone resistant derivative of the HL-60 cell line (see ATCC CCL-240) which was obtained from peripheral blood leukocytes obtained by leukopheresis from patient with acute promyelocytic leukemia. HL-60/MX1 (see ATCC CRL-2258) cells were selected and subcloned in 1987 for resistance to 39 nM mitoxantrone, an anthracenedione antitumor agent. Compare HL-60 from ATCC on Biocompare.com. HL-60 is a promyelocytic cell line derived by S.J. Collins, et al.

No. 15338-100). Important Guidelines for Transfection Follow these important guidelines when transfecting DNA into HL-60 cells using Lipofectamine™ LTX Reagent: Purpose. The screening procedure is called the NCI-60 Human Tumor Cell Lines Screen, and it is one of the Discovery & Development Services of NCI's Developmental Therapeutics Program (DTP). The screening rates for each cell line the cytostatic and cytotoxic impact of tested substances.. Due to the diversity of the cell lines, it is possible to compare tested compounds … Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months. This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with butyric acid.

1250.

token nektaru ethfinex
co znamenají fiat peníze
api omezuje aws
jaderná bomba v severní koreji
proč byla světová banka připravena kvíz

Compare HL-60 from ATCC on Biocompare.com. HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.

A Hematoxylen-Eosin stained paraffin section of a subcutaneous HL-60 xenograft is shown on the right. As routine quality controls, the cells are regularly checked for I usually replenish my stock of HL-60 cells from ATCC when I encounter problems with differentiation. Hope this helps, Paige. Cite. 10th Sep, 2014. Steven J Ackerman. University of Illinois at HL-60 cells were obtained from Dr Dallas Hyde (University of California, Davis, USA) or ATCC (Manassas, VA, USA) and maintained in RPMI 1640 supplemented with 10% foetal calf serum.

HL-60 cells to grow healthy need higher con. of serum (20%) and THP-1 cells need mercaptoethanol containing medium but 10% serum. if you add 1uM Phorbol myristate acetate, THP-1 cell will attach

Gray columns show the coculture of S. aureus ATCC 25923 with HL-60 cells differentiated with DMF and activated with phorbol 12-myristate 13-acetate (PMA). Values are means and SD of three independent experiments ( *** p ATCC Genome Portal. The ATCC Genome Portal makes it easy to find the high-quality whole-genome sequencing data needed for your research. With a free account, you can easily search, download, and analyze hundreds of high-quality genomes. HL-60 (ATCC® CCL-240™). HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, The HL-60 cell line is a human acute myeloid leukemia cell line.

Handling Procedure for Frozen Cells. Page 1 of 3. Product Sheet. HL60 (ATCC® CCL240™). HL-60-Luc2 (ATCC® CCL-240-LUC2™). This luciferase expressing cell line was derived from parental line CCL-240 by transduction with lentiviral vector The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates Part of: MYC genetic alteration cell panel (ATCC TCP-1035).